Protein_Domain

Part:BBa_K1128008:Design

Designed by: Mahamad Charawi   Group: iGEM13_Penn   (2013-10-28)

Methyl CpG Binding Protein 2 with C-Terminus Cut off


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 158
    Illegal PstI site found at 836
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 158
    Illegal PstI site found at 836
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 158
    Illegal PstI site found at 836
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 158
    Illegal PstI site found at 836
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 591
    Illegal SapI.rc site found at 642

Design Notes

We are currently in the process of optimizing this part for use in biobrick cloning by making it compatible with biobrick assemble standards, and will submit to the registry when it is complete.




http://s11.postimg.org/4jpxrunvn/mecp2.jpg
A SDS-Page gel shows that the a c terminus truncation of MECP2 gene is expressed.





Source

See BBa_K1128007.

References